DNA Extraction and Purification
Cayman Chemical Fungal Lysergol 250mg
An ergot alkaloid and 5-HT1A, 5-HT2A, and 5-HT2C receptor agonist (EC50s = 73, 1.6, and 6.6 nM, respectively, in a FLIPR assay); induces contractions in isolated rat tail arteries (EC50 = 436.5 nM); enhances the antibacterial activity of nalidixic acid against susceptible and drug-resistant E. coli at 10 UG/ml
Biochain Institute Inc Genomic DNA - Alzheimer's Disease: Brain, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Human Tumor Cell Line: Raji, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
New England Biolabs, Inc. Monarch® gDNA Purification Columns - 100 columns
The Monarch gDNA Purification Columns are a component of the Monarch Genomic DNA Purification Kit and can be used to purify up to 30 ug genomic DNA from a variety of sample types. They are available for purchase separately for your convenience and supplied with Monarch Collection Tubes II.
- A component of the Monarch Genomic DNA Purification Kit
- Can be used to purify up to 30 ug genomic DNA from a variety of sample types
- Contains 100 columns and 100 collection tubes
- Compatible with Monarch Collection Tubes II
- Monarch kit components are also available separately for added convenience
New England Biolabs, Inc. NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® – 24 reactions
The Ultra II Directional RNA Library Prep Kit for Illumina delivers significantly increased sensitivity and specificity from your RNA-seq experiments, from ever-decreasing amounts of input RNA. In conjunction with ribosomal RNA (rRNA) depletion or poly(A) enrichment, the kit enables the production of high quality libraries from 5 ng or 10 ng of Total RNA, respectively, up to 1 µg. The NEBNext Ultra II Directional RNA Library Prep Kit derives its directionality from the “dUTP” method for strand-specificity, with proven superiority for this application.
- Generate the highest yields of high quality libraries, with a broad range of input amounts
- Increase the complexity and transcript coverage of your libraries
- Optimize your time with streamlined workflows, reduced hands-on time, and automation compatibility
- Rely on robust performance, even with low quality RNA, including FFPE
Zymo Research Corporation DNA/RNA Shield Lysis Tube (2 mm) (Tissue) (50 pack)
DNA/RNA Shield Lysis and Collection tubes are designed for the streamlined collection, transport, and processing of biological specimen. They are pre-filled with DNA/RNA Shield and a lysis matrix tailored for tough-to-lyse- sample inputs from tissue to microbial samples. Collected samples do not require additional handling for processing and are ready for homogenization.
Biochain Institute Inc Genomic DNA - Human Diabetic Diseased Tissue: Adrenal, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Emphysema: Lung, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Qiagen, Inc. QIAquick Gel Extraction Kit, for gel extraction or cleanup of 50 reactions
Kit; Qiagen QIAquick Gel Extraction Kit; Contains: 50 QIAquick Spin Columns, buffers, collection tubes, comprehensive handbook
Boca Scientific Inc GeneFiX Saliva-Prep 2 Isolation Kit, 50 Reactions
Isohelix GeneFiX Saliva-Prep 2 Isolation kit is specifically designed for use with saliva collection samples. It uses a new formulated precipitation method to quickly and easily isolate the DNA, up to volumes of 2ml from the GeneFix Saliva Collectors. The GeneFiX Saliva-Prep 2 kit does not use solvents, columns or filtration. The reduced number of protocol steps makes it extremely easy-to-use when a simple yet efficient isolation method is required.
Supply Solutions Roche - Biotin-16-dUTP, Solution; pkg of 50 uL (50 nmol; 1mM)
Roche - Biotin-16-dUTP, Solution; pkg of 50 uL (50 nmol; 1mM)
Cygnus Technologies CHO Host Cell DNA Detection Kit in Tubes
Used to measure residual CHO host cell DNA, this kit uses a 2 ml microfuge tube format and includes our proprietary extraction procedure, which does not use iodine or glycogen. The superior co-precipitating reagents do not interfere with qPCR and ensure that pellets do not become dislodged during extraction. This eliminates the need to centrifiuge samples or repeat a run. Cell line-specific, CHO DNA calibrators are included in the kit. Upon completion of extraction, samples are reacted with PicoGreen solution. Time to completion is under 2 hours. Each kit supplies enough reagent for the analysis of 50 samples in duplicate.
Zymo Research Corporation EZ-96 DNA Methylation™ Kit (Shallow-Well) (2 x 96 Rxns)
The EZ-96 DNA MethylationTM Kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite where cytosine is converted into uracil. The products innovative desulphonation technology eliminates otherwise cumbersome precipitations.The kit is designed to reduce template degradation, minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.